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MB Sample ID: SA014474
| Local Sample ID: | SA015 |
| Subject ID: | SU000337 |
| Subject Type: | Bacterial cells |
| Subject Species: | Bacteria |
| Species Group: | Microorganism |
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Sample Preparation:
| Sampleprep ID: | SP000345 |
| Sampleprep Summary: | 1. After quenching the cells add 1 X 106 dried cells to 1.5 ml eppendorff tube. 2. Place the eppendorf tube with cells on dry ice for 20 minutes or til the cells are completely frozen and ice for 20 minutes, till they are completely thawed. Repeat this step twice. 3. Add 1ml of extraction solvent which has been pre-chilled using the ThermoElectron Neslab RTE 740 cooling bath set to -20°C. to the ependorff tube with cells. 4. Repeat step 2 twice with the extraction solvent. 5. Vortex the sample for 10s and shake for 5min at 4°C using the Orbital Mixing Chilling/Heating plate. Centrifuge samples for 2min at 14000 rcf using the centrifuge Eppendorf 5415 D. 6. Aliquot two 500µL portions of the supernatant. One for analysis and one for backup. Store one aliquot in the -20°C freezer as a backup. 7. Evaporate one 500µL aliquot of the sample in the Labconco Centrivap cold trap concentrator to complete dryness. 8. Submit to derivatization. |
| Sampleprep Protocol Filename: | SOP_Extraction_of_Cell_pellets.pdf |
