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MB Sample ID: SA020544
| Local Sample ID: | ciMB1_D11b |
| Subject ID: | SU000435 |
| Subject Type: | Cells |
| Subject Species: | Plasmodium falciparum |
| Taxonomy ID: | 5833 |
| Genotype Strain: | 3D7 |
| Species Group: | Microorganism |
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Sample Preparation:
| Sampleprep ID: | SP000442 |
| Sampleprep Summary: | Culture medium was removed by aspiration, and the metabolism of the settled iRBCs quenched by addition of ice-cold phosphate buffered saline (PBS). Subsequent steps were performed on ice. Cells were pelleted by centrifugation for 5 min at 1000 × g and the PBS supernatant removed prior to the addition of 135 µL methanol (containing internal standard compounds: CHAPS, CAPS and PIPES) and rapid mixing by pipetting three times to extract iRBC metabolites. Samples were left on ice with gentle agitation for 60 minutes, then centrifuged at 3000 × g to remove the insoluble material. Supernatants were transferred to glass HPLC vials and stored (< 4 months) at -80 °C until analysis. |
| Processing Method: | Lysis with organic solvent and mixing at 4°C |
| Processing Storage Conditions: | on ice or 4°C |
| Extraction Method: | Methanol (10:1) |
| Extract Storage: | -80°C |
| Sample Spiking: | internal standards (CHAPS, CAPS, PIPES all at 1 µM) mixed in extraction solvent |
| Cell Type: | Plasmodium falciparum-infected red blood cells (8% parasitaemia and 3% haematocrit |
