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MB Sample ID: SA022043

Local Sample ID:S_81
Subject ID:SU000459
Subject Type:Human
Subject Species:Homo sapiens
Taxonomy ID:9606
Gender:Female
Human Race:African American
Human Ethnicity:African American
Species Group:Human

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Sample Preparation:

Sampleprep ID:SP000466
Sampleprep Summary:Aliquots of each de-identified sample were shipped to the NIH RTI-RCMRC on dry ice and immediately stored at -80 °C after being logged in for metabolomics analysis. A total of 48 study samples were weighed on dry ice to confirm weights and approximately 50 mg of the tissue was transferred to labeled MagNa Lyser bead tubes on ice and ice cold 50:50 acetonitrile:water was added, and samples were homogenized with two 30sec pulses at 3000rpm. Tubes were centrifuged at 16,000 rcf for 10 minutes at room temperature and supernatants were transferred to 1.5mL pre-labeled LoBind Eppendorf tubes. Aliquots of 500uL were then transferred into labeled 2.0mL LoBind Eppendorf tubes. Analytical quality control (QC) whole study pool samples were generated by transferring an additional 125µL aliquot of each study sample into a 10mL cyrovial and vortexed. To generate Total Pooled QC samples 500uL was transferred to 5 labeled 2.0mL LoBind Eppendorf tubes. All samples were lyophilized to complete dryness overnight, then reconstituted with 700uL of NMR Master Mix solution containing Chenomx ISTD: DSS-d6 and Phosphate Buffer at 7.4 pH. The tubes were vortexed for 4 min on a multi-tube vortexer and centrifuged at 16,000 rcf for 5 min. A 600uL aliquot of supernatants were transferred into a pre-labeled 5mm 4 NMR tubes for data acquisition on a 700 MHz spectrometer.
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