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MB Sample ID: SA022621
Local Sample ID: | WBB2-1 |
Subject ID: | SU000465 |
Subject Type: | Human |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Species Group: | Human |
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Sample Preparation:
Sampleprep ID: | SP000472 |
Sampleprep Summary: | Bursting bubbles were generated at ambient conditions by feeding gas (1-100 kPa; 1-100 L/s) through an air diffuser at the bottom of diluted (200 times in ultrapure water) urine solution (50-500 mL). Diffusers with three different pore sizes were tested: 100 µm; 1-10 µm; 0.2-5 µm. The first diffuser was made in house by pulling 10 fused silica capillaries with i.d. 100 µm through a rubber piece. The second diffuser was made from porous ceramics and was purchased from Shiyuan Appliances, Shenzhongshan, Guangdong, China. The third diffuser was made from porous ceramics and was purchased from Junfeng Tradnig Ltd., Ningbo, Zhejiang, China. The bursting bubble aerosol was collected using a slanted glass slide fixed above the liquid surface. Typically, ca. 100 µL of aerosol was collected within 5-10 min from 500 mL bulk solution at the gas pressure of 10 kPa. |