Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA022895

Local Sample ID:	UCSF-01029
Subject ID:	SU000473
Subject Type:	Animal
Subject Species:	Mus musculus
Taxonomy ID:	10090
Species Group:	Mammal

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Sample Preparation:

Sampleprep ID:	SP000480
Sampleprep Summary:	sample extract reconstituted in solvents containing a series of standards at fixed concentrations to ensure injection and chromatographic consistency. One aliquot was analyzed using acidic positive ion conditions, chromatographically optimized for more hydrophilic compounds. The extract was gradient eluted from a C18 column (Waters UPLC BEH C18-2.1 × 100 mm, 1.7 μm) using water and methanol, containing 0.05 % perfluoropentanoic acid (PFPA) and 0.1 % formic acid (FA). Another aliquot was also analyzed using acidic positive ion conditions,however it was chromatographically optimized for more hydrophobic compounds. In this method, the extract was gradient eluted from the same C18 column using methanol, acetonitrile, water, 0.05 % PFPA and 0.01 % FA and was operated at an overall higher organic content. Another aliquot was analyzed using basic negative ion optimized conditions using a separate dedicated C18 column. The basic extracts were gradient eluted from the column using methanol and water

Sampleprep ID:

SP000480

Sampleprep Summary:

sample extract reconstituted in solvents containing a series of standards at fixed concentrations to ensure injection and chromatographic consistency. One aliquot was analyzed using acidic positive ion conditions, chromatographically optimized for more hydrophilic compounds. The extract was gradient eluted from a C18 column (Waters UPLC BEH C18-2.1 × 100 mm, 1.7 μm) using water and methanol, containing 0.05 % perfluoropentanoic acid (PFPA) and 0.1 % formic acid (FA). Another aliquot was also analyzed using acidic positive ion conditions,however it was chromatographically optimized for more hydrophobic compounds. In this method, the extract was gradient eluted from the same C18 column using methanol, acetonitrile, water, 0.05 % PFPA and 0.01 % FA and was operated at an overall higher organic content. Another aliquot was analyzed using basic negative ion optimized conditions using a separate dedicated C18 column. The basic extracts were gradient eluted from the column using methanol and water