Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA025685

Local Sample ID:	Sa_pro_39
Subject ID:	SU000517
Subject Type:	Human
Subject Species:	Homo sapiens
Taxonomy ID:	9606
Species Group:	Human

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Sample Preparation:

Sampleprep ID:	SP000524
Sampleprep Summary:	Frozen saliva samples were freeze-dried overnight using a freeze-dryer (VD-800F; TAITEC, Saitama, Japan), and metabolites were extracted with 1 ml of MeOH/H2O/CHCl3 (5/2/2, v/v/v). As an internal standard, 60 µl of ribitol (0.2 mg/ml) was added to the mixture. The sample was centrifuged at 16,000 g for 3 min at 4 °C, and the supernatant (900 µl) was transferred to another microtube and mixed with 400µl of water. After centrifugation at 16,000 g for 3 min at 4 °C, 800µl of the supernatant was transferred to another microtube whose cap was pierced. The extract was evaporated using a vacuum centrifuge dryer for 2 h in order to remove methanol, then freeze-dried overnight. For derivatization, 100 µl of methoxyamine hydrochloride (20 mg/ml in pyrimidine) and 50 µl of N-methyl-N-(trimethylsilyl)trifluoroacetamide were employed. The sample was then used for GCMS analysis, and a 1 µL of sample was injected in split mode (25/1, v/v).

Sampleprep ID:

SP000524

Sampleprep Summary:

Frozen saliva samples were freeze-dried overnight using a freeze-dryer (VD-800F; TAITEC, Saitama, Japan), and metabolites were extracted with 1 ml of MeOH/H2O/CHCl3 (5/2/2, v/v/v). As an internal standard, 60 µl of ribitol (0.2 mg/ml) was added to the mixture. The sample was centrifuged at 16,000 g for 3 min at 4 °C, and the supernatant (900 µl) was transferred to another microtube and mixed with 400µl of water. After centrifugation at 16,000 g for 3 min at 4 °C, 800µl of the supernatant was transferred to another microtube whose cap was pierced. The extract was evaporated using a vacuum centrifuge dryer for 2 h in order to remove methanol, then freeze-dried overnight. For derivatization, 100 µl of methoxyamine hydrochloride (20 mg/ml in pyrimidine) and 50 µl of N-methyl-N-(trimethylsilyl)trifluoroacetamide were employed. The sample was then used for GCMS analysis, and a 1 µL of sample was injected in split mode (25/1, v/v).