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MB Sample ID: SA026895
| Local Sample ID: | 2023623 |
| Subject ID: | SU000537 |
| Subject Type: | Animal |
| Subject Species: | Macaca mulatta |
| Taxonomy ID: | 9544 |
| Age Or Age Range: | N/A| N/A| ~3years| ~3years| ~3years| ~3years| ~3years |
| Weight Or Weight Range: | N/A| N/A| 6.05 kg| 6.05 kg| 6.05 kg| 6.05 kg| 6.05 kg |
| Gender: | Male| Male| Male| Male| Male| Male| Male |
| Subject Comments: | Transfusion Donor| Reference Animal| Experimental Subject|Experimental Subject| Experimental Subject| Experimental Subject| Experimental Subject |
| Species Group: | Mammal |
Select appropriate tab below to view additional metadata details:
Sample Preparation:
| Sampleprep ID: | SP000544 |
| Sampleprep Summary: | aliquots of 65ul sample were mixed with 130ul acetonitrile and 3.25ul internal standard. Keep on ice and vortex every 15 minutes. Centrifuge for 10 minutes at 13.2rpm and 4C. Using a pipette, 130ul of supernatant was removed and placed into autosampler vials for the mass spectrometer. |
| Sampleprep Protocol Filename: | Metab_Sample_Preparation_Plasma_serum_v1_May_17_2013.docx E4_E3_E18_E23_E24_E25_MalariaCore_Metabolomics_SOP_v1_6October2016.docx |
| Processing Method: | Precipitation of protein, centrifuge, and remove supernatant |
| Processing Storage Conditions: | on ice |
| Extraction Method: | 1:2 sample:acetonitrile |
| Extract Storage: | Pipette supernatant into autosampler vials for mass spectrometer |
| Sample Spiking: | C18 standards: Caffeine and Diethyl-toluamide. Stable isotopes: [13C6]-D-glucose, [15N]-indole, [1,2-13C2]-palmitic acid, [15N,13C5]-L-methionine, [2-15N]-L-lysine dihydrochloride, [15N]-choline chloride, [13C5]-L-glutamic acid, [13C7]-benzoic acid, [15N]-L-tyrosine, [15N2]-uracil, [3,4-13C2]-cholesterol, [3,3-13C2]-cystine, [trimethyl-13C3]-caffeine, [U-13C5, U-15N2]-L-glutamine. |
