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MB Sample ID: SA028151
| Local Sample ID: | S_60exp3 |
| Subject ID: | SU000568 |
| Subject Type: | Cells |
| Subject Species: | Plasmodium falciparum |
| Taxonomy ID: | 5833 |
| Genotype Strain: | Cam3.IIR539T (Cambodian isolate, artemisinin resistant) R1 | Cam3.IIC580Y (Cambodian isolate, artemisinin resistant) R2 | Cam3.IIrev (Cambodian isolate, artemisinin sensitive) S | uninfected red blood cells |
| Cell Strain Details: | Artemisinin resistant strain (R1) | Artemisinin resistant strain (R2) | Artemisinin sensitive strain (S) | uninfected red blood cells |
| Cell Primary Immortalized: | Primary | Primary | Primary | Primary |
| Cell Counts: | 5 x 10e7 per sample (purified infected cells) | 5 x 10e7 per sample (purified infected cells) | 5 x 10e7 per sample (purified infected cells) | 5 x 10e7 per sample |
| Species Group: | Microorganism |
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Sample Preparation:
| Sampleprep ID: | SP000575 |
| Sampleprep Summary: | Metabolism was quenched by washing with ice cold PBS (1 mL). Cells were then pelleted by centrifugation (6000 xg for 3 min) and metabolites were extracted from 5 x 107 uninfected red blood cells and 5 x 107 parasitised red blood cells reticulocytes by addition of 140 µL methanol containing internal standards (CHAPS, CAPS, PIPES and TRIS; 1 µM) and left for 1 hour at 4 °C with automatic vortex. After mixing, cellular debris was removed by centrifugation (16,000 rpm for 10 mins) and the supernatant was kept at -20 °C prior to analysis. |
| Processing Method: | Lysis with automatic vortex at 4°C |
| Processing Storage Conditions: | On ice or 4°C |
| Extraction Method: | Methanol |
| Extract Storage: | -20°C |
| Sample Spiking: | Internal standards (CHAPS, CAPS, PIPES and TRIS; all at 1 µM) mixed in extraction solvent |
| Cell Type: | Artemisinin resistant and sensitive parasitised red blood cells and uninfected red blood cells |
