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MB Sample ID: SA028319
| Local Sample ID: | QC005 |
| Subject ID: | SU000570 |
| Subject Type: | Cells |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Species Group: | Human |
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Sample Preparation:
| Sampleprep ID: | SP000577 |
| Sampleprep Summary: | Supernatants were combined and evaporated to dryness. Samples were then re-suspended in 200 µL LC-MS-grade water. A 2 mL AG-1 X8 100-200 anion exchange resin (Bio-Rad) was washed with 5 column volumes (10 mL) of 3 N HCl followed by transfer of re-suspended extracts to resin column. Metabolites were eluted using 10 mL 3 N HCl. Samples were evaporated to dryness and re-suspended in 100 µL MTBSTFA/ACN (1:1, v/v) and shaken at 60°C for 1 hour. Derivatized extracts were further diluted (1:4) with a MTBSTFA/ACN (1:1, v/v) mixture and transferred to glass vials with micro-inserts and capped immediately. |
| Sampleprep Protocol Filename: | The common feature of IDH1 and IDH2 mutations.pdf |
