Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA028528

Local Sample ID:	19
Subject ID:	SU000572
Subject Type:	Healthy males
Subject Species:	Homo sapiens
Taxonomy ID:	9606
Age Or Age Range:	20-30
Gender:	Men
Human Ethnicity:	Caucasian and south asian
Species Group:	Human

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Sample Preparation:

Sampleprep ID:	SP000579
Sampleprep Summary:	Targeted metabolomics Metabolomic measurements were performed on serum samples taken at thermoneutral and cold conditions at the Genome Analysis Center at the Helmholtz Zentrum, Munich, Germany, using the Biocrates AbsoluteIDQTM p150 kit (BIOCRATES Life Science AG, Innsbruck, Austria) and ESI-FIA-MS/MS measurements (Menni et al. 2013). The assay allows simultaneous quantification of 163 small molecule metabolites within 10 µL serum. The assay includes free carnitine, 40 acylcarnitines, 14 amino acids (13 proteinogenic + ornithine), hexoses (of which approx. 90-95% is glucose), 92 glycerophospholipids including 15 lysophosphatidylcholines (LysoPCs) and 77 phosphatidylcholines (PCs), and 15 sphingolipids (Menni et al. 2013; Illig et al. 2010). Quantification of the metabolites was achieved by reference to appropriate internal standards. The assay has been previously described (Menni et al. 2013; Illig et al. 2010).

Sampleprep ID:

SP000579

Sampleprep Summary:

Targeted metabolomics Metabolomic measurements were performed on serum samples taken at thermoneutral and cold conditions at the Genome Analysis Center at the Helmholtz Zentrum, Munich, Germany, using the Biocrates AbsoluteIDQTM p150 kit (BIOCRATES Life Science AG, Innsbruck, Austria) and ESI-FIA-MS/MS measurements (Menni et al. 2013). The assay allows simultaneous quantification of 163 small molecule metabolites within 10 µL serum. The assay includes free carnitine, 40 acylcarnitines, 14 amino acids (13 proteinogenic + ornithine), hexoses (of which approx. 90-95% is glucose), 92 glycerophospholipids including 15 lysophosphatidylcholines (LysoPCs) and 77 phosphatidylcholines (PCs), and 15 sphingolipids (Menni et al. 2013; Illig et al. 2010). Quantification of the metabolites was achieved by reference to appropriate internal standards. The assay has been previously described (Menni et al. 2013; Illig et al. 2010).