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MB Sample ID: SA029938
| Local Sample ID: | 5_C4 |
| Subject ID: | SU000592 |
| Subject Type: | Animal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Genotype Strain: | C57BL/6 |
| Species Group: | Mammal |
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Sample Preparation:
| Sampleprep ID: | SP000599 |
| Sampleprep Summary: | CE-TOFMS-based metabolome analysis(sample preparation) Fecal samples were freeze dried, and disrupted by vigorous shaking at 1,500 rpm for 10 min with four 3 mm zirconia beads by Shake Master NEO (Bio Medical Science Inc.). 10 mg (±0.5 mg) fecal samples were homogenized with 500 μl MeOH containing internal standards (20 μM each of methionine sulfone, and D-camphor-10-sulfonic acid (CSA)) and 100 mg of 0.1 mm and four of 3 mm zirconia/silica beads (BioSpec Products). After vigorous shaking (1,500 rpm for 5 min) by Shake Master NEO (Bio Medical Science Inc.), 200 μl of Milli-Q water and 500 μl of chloroform was added and then shaking in a same manner as before. After centrifugation at 4,600 × g for 15 min at 4°C, the supernatant was transferred to a 5kDa cutoff centrifugal filter tube. The filtrate was centrifugally concentrated at 40°C and reconstituted with 40 μl of Milli-Q water. |
| Sampleprep Protocol Filename: | Sample_Prep_Protocol.pdf |
| Processing Method: | Homogenized with 500 μl MeOH containing internal standards and 100 mg of 0.1 mm and four of 3 mm zirconia/silica beads |
| Processing Storage Conditions: | On ice |
| Extraction Method: | Chloroform |
| Extract Cleanup: | 5kDa cutoff centrifugal filter tube |
| Sample Resuspension: | 40 μl of Milli-Q water |
