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MB Sample ID: SA032624

Local Sample ID:1941204
Subject ID:SU000615
Subject Type:Animal
Subject Species:Macaca mulatta
Taxonomy ID:9544
Genotype Strain:N/A|6.53kg|6.87kg|6.09kg|7.23kg|6.48kg
Gender:Male| Male| Male| Male| Male| Male
Animal Animal Supplier:none|Yerkes Field Station|Yerkes Primate Research Center|Yerkes Primate Research Center| |Yerkes Primate Research Center
Animal Housing:Metallic|Metallic|Metallic|Metallic|Metallic|Metallic
Animal Light Cycle:12/11|12/12|12/12|12/12|12/12|12/12
Animal Feed:Monkey Jumbo Diet 5037 and 5038
Animal Water:tap in bottle
Animal Inclusion Criteria:Weight
Species Group:Mammal

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Sample Preparation:

Sampleprep ID:SP000622
Sampleprep Summary:aliquots of 65ul sample were mixed with 130ul acetonitrile and 3.25ul internal standard. Keep on ice for 30 minutes and vortex every 15 minutes. Centrifuge for 10 minutes at 13.2rpm and 4C. Using a pipette, 130ul of supernatant was removed and placed into autosampler vials for the mass spectrometer.
Sampleprep Protocol Filename:Metab_Sample_Preparation_Plasma_serum_v1_May_17_2013.docx
Processing Method:Precipitation of protein, centrifuge, and remove supernatant
Processing Storage Conditions:On ice
Extraction Method:1:2 sample:acetonitrile
Extract Storage:Pipette supernatant into autosampler vials for mass spectrometer
Sample Spiking:C18 standards: Caffeine and Diethyl-toluamide. Anion Exchange Standards: Cystine, cysteine-glutathione disulfide, carboxymethyl-cysteine, glutathione disulfide, carboxymethyl-glutathione. Stable isotopes: [13C6]-D-glucose, [15N]-indole, [1,2-13C2]-palmitic acid, [15N,13C5]-L-methionine, [2-15N]-L-lysine dihydrochloride, [15N]-choline chloride, [13C5]-L-glutamic acid, [13C7]-benzoic acid, [15N]-L-tyrosine, [15N2]-uracil, [3,4-13C2]-cholesterol, [3,3-13C2]-cystine, [trimethyl-13C3]-caffeine, [U-13C5, U-15N2]-L-glutamine.
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