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MB Sample ID: SA032837
| Local Sample ID: | 2140077 |
| Subject ID: | SU000622 |
| Subject Type: | Animal |
| Subject Species: | Macaca mulatta |
| Taxonomy ID: | 9544 |
| Weight Or Weight Range: | NA|6.94kg|9.04kg|8.03kg|8.83kg|8.68kg |
| Gender: | Male|Male|Male|Male|Male|Male |
| Animal Animal Supplier: | Yerkes Field Station|Yerkes Field Station|Yerkes Primate Research Center|Yerkes Primate Research Center||Yerkes Primate Research Center |
| Animal Housing: | Metallic|Metallic|Metallic|Metallic|Metallic|Metallic |
| Animal Light Cycle: | 12/12|12/12|12/12|12/12|12/12|12/12 |
| Animal Feed: | Monkey Jumbo Diet 5037 and 5038|Monkey Jumbo Diet 5037 and 5038|Monkey Jumbo Diet 5037 and 5038|Monkey Jumbo Diet 5037 and 5038|Monkey Jumbo Diet 5037 and 5038|Monkey Jumbo Diet 5037 and 5038 |
| Animal Water: | tap in bottle|tap in bottle|tap in bottle|tap in bottle|tap in bottle|tap in bottle |
| Animal Inclusion Criteria: | Weight|Weight|Weight|Weight|Weight|Weight |
| Species Group: | Mammal |
Select appropriate tab below to view additional metadata details:
Sample Preparation:
| Sampleprep ID: | SP000629 |
| Sampleprep Summary: | aliquots of 30ul sample were mixed with 100ul acetonitrile and 2.5ul internal standard. Keep on ice for 30 minutes and vortex every 15 minutes. Centrifuge for 10 minutes at 13.2rpm and 4C. Using a pipette, 100ul of supernatant was removed and placed into autosampler vials for the mass spectrometer. |
| Sampleprep Protocol Filename: | E03_MB_Sample_Preparations_Plasma_serum_HFQE_V1_3282016.docx, E4_E3_E18_E23_E24_E25_MalariaCore_Metabolomics SOP_v1_6October2016.docx |
| Processing Method: | Precipitation of protein, centrifuge, and remove supernatant |
| Processing Storage Conditions: | On ice |
| Extraction Method: | 1:2 sample:acetonitrile |
| Extract Storage: | Pipette supernatant into autosampler vials for mass spectrometer |
| Sample Spiking: | C18 Standards: Caffeine, Diethyl-toluamide. Internal Standards/Stable Isotopes: [13C6]-D-glucose, [15N]-indole, [15N,13C5]-L-methionine, [2-15N]-L-lysine dihydrochloride, [13C5]-L-glutamic acid, [15N]-L-tyrosine, [15N2]-uracil, [3,3-13C2]-cystine, [trimethyl-13C3]-caffeine, [U-13C5, U-15N2]-L-glutamine |
