Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA033153

Local Sample ID:	22753T
Subject ID:	SU000624
Subject Type:	Human Plasma
Subject Species:	Homo sapiens
Taxonomy ID:	9606
Age Or Age Range:	57-70
Gender:	Males and Females
Human Race:	Non-Hispanic White
Human Smoking Status:	Current and Former Smokers
Species Group:	Human

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Sample Preparation
Chromatography
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MS

Sample Preparation:

Sampleprep ID:	SP000631
Sampleprep Summary:	100µL of plasma was prepared using methanol to extract precipitate the proteins into a pellet. A liquid-liquid extraction with water and methyl-tert butyl ether (MTBE) was used to partition the supernatant into an aqueous layer and an organic layer (lipids). The aqueous layer was dried down in a SpeedVac at 45°C, then reconstituted in 100µL of 95:5 water:acetonitrile. The lipid fraction was dried down in a SpeedVac at 45°C, then reconstituted in 200µL of 100% methanol.

Sampleprep ID:

SP000631

Sampleprep Summary:

100µL of plasma was prepared using methanol to extract precipitate the proteins into a pellet. A liquid-liquid extraction with water and methyl-tert butyl ether (MTBE) was used to partition the supernatant into an aqueous layer and an organic layer (lipids). The aqueous layer was dried down in a SpeedVac at 45°C, then reconstituted in 100µL of 95:5 water:acetonitrile. The lipid fraction was dried down in a SpeedVac at 45°C, then reconstituted in 200µL of 100% methanol.