Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA046918

Local Sample ID:	PLAZ-21
Subject ID:	SU000872
Subject Type:	Animal
Subject Species:	Macaca mulatta
Taxonomy ID:	9544
Species Group:	Mammal

Select appropriate tab below to view additional metadata details:

Sample Preparation:

Sampleprep ID:	SP000879
Sampleprep Summary:	Oxylipins, endocannabinoids, bile acids and fatty acids were isolated from an aliquot of 20μl plasma in Eppendorf tubes. The tube was spiked was 5ul of OxyEndo Fusion, 10μl spike of Bile Acid SSTD Solutions, and 5μl of BHT/EDTA solution. A total of 100μl CUDA/PHAU in methanol was added to the tube, before it was capped, vortexed, and centrifuged (3 min, 10,000 RCF, room temp). It was then placed on wet ice for 5 min. Next it was spin filtered with Millapore spin filters (0.1 µm, Millipore, Billerica, MA) at 8 ºC, 4500 RPM for 3 min, before being transferred to 2 mL LC-MS amber vials. Extracts were stored at -20ºC until analysis by UPLC-MS/MS. The internal standard was used to quantify the recovery of surrogate standards.

Sampleprep ID:

SP000879

Sampleprep Summary:

Oxylipins, endocannabinoids, bile acids and fatty acids were isolated from an aliquot of 20μl plasma in Eppendorf tubes. The tube was spiked was 5ul of OxyEndo Fusion, 10μl spike of Bile Acid SSTD Solutions, and 5μl of BHT/EDTA solution. A total of 100μl CUDA/PHAU in methanol was added to the tube, before it was capped, vortexed, and centrifuged (3 min, 10,000 RCF, room temp). It was then placed on wet ice for 5 min. Next it was spin filtered with Millapore spin filters (0.1 µm, Millipore, Billerica, MA) at 8 ºC, 4500 RPM for 3 min, before being transferred to 2 mL LC-MS amber vials. Extracts were stored at -20ºC until analysis by UPLC-MS/MS. The internal standard was used to quantify the recovery of surrogate standards.