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MB Sample ID: SA047812
| Local Sample ID: | 16_Sample_90_082914 |
| Subject ID: | SU000892 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Species Group: | Human |
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Sample Preparation:
| Sampleprep ID: | SP000899 |
| Sampleprep Summary: | Metabolite extraction Plasma metabolites were extracted by adding 1mL of a working solution composed of acetonitrile, isopropanol and water (3:3:2) containing isotope-labeled internal standards at a concentration of 1.25 μg/mL (Tyrosine-d2, L-glutamic-2,3,3,4,4-d5 acid, L-alanine-2,3,3,3-d4, L-phenyl-d5-alanine-2,3,3,-d3, Glycine-d5, Myristic acid d27) to 30μL of plasma in order to evaluate the quality of metabolites extraction. After vortexing, samples were centrifuged at 14,500g for 15 minutes at room temperature. Each supernatant was then concentrated to dryness in speedvac. The dried samples were kept at -20°C until derivatization prior to analysis by GC-MS. 20μL of a 20mg/mL methoxyamine hydrochloride in pyridine was added to the dried extracts, vortexed and incubated at 30°C for 90 minutes. After returning the samples at room temperature, 80μL of MSTFA was added, vortexed and incubated at 30°C for 30 minutes. Samples were then centrifuged at 14,500rpm for 15 minutes, and 60μL of the supernatant was transferred into 250μL clear glass autosampler vials. |
| Sampleprep Protocol ID: | 3_SamplePrep |
