Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA051572

Local Sample ID:	215_AIR_PRE
Subject ID:	SU000923
Subject Type:	Human
Subject Species:	Homo sapiens
Taxonomy ID:	9606
Weight Or Weight Range:	Normal/Overweight/Obese
Gender:	Male/Female
Human Race:	Black/White
Species Group:	Human

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Sample Preparation
Chromatography
Analysis
MS

Sample Preparation:

Sampleprep ID:	SP000930
Sampleprep Summary:	Aliquots of 30µL plasma were mixed with 1mL 3:3:2 ACN:IPA:H2O by vortex mixer then centrifuged. Supernatant recovered. Archived 450µL of supernatant. Continued prep of remaining 450µL supernatant. Evaporated to dryness w/speed vac. Formation of methoximes by adding 10µL of 40mg/mL MeOx in pyridine to each sample. Placed onto Thermomixer for 90 min at 30⁰C. Then FAME retention index markers and MSTFA added to each sample for derivatization step. Samples returned to Thermomixer for 45 min at 70⁰C.

Sampleprep ID:

SP000930

Sampleprep Summary:

Aliquots of 30µL plasma were mixed with 1mL 3:3:2 ACN:IPA:H2O by vortex mixer then centrifuged. Supernatant recovered. Archived 450µL of supernatant. Continued prep of remaining 450µL supernatant. Evaporated to dryness w/speed vac. Formation of methoximes by adding 10µL of 40mg/mL MeOx in pyridine to each sample. Placed onto Thermomixer for 90 min at 30⁰C. Then FAME retention index markers and MSTFA added to each sample for derivatization step. Samples returned to Thermomixer for 45 min at 70⁰C.