Return to study ST000903 main page
MB Sample ID: SA052931
Local Sample ID: | Beta_10_7 |
Subject ID: | SU000940 |
Subject Type: | Animal |
Subject Species: | Rattus norvegicus |
Taxonomy ID: | 10116 |
Genotype Strain: | Sprague-Dawley |
Weight Or Weight Range: | 180-220 g |
Gender: | Male |
Animal Animal Supplier: | Beijing Huafukang Bioscience |
Animal Light Cycle: | 12:12 light-dark cycle |
Animal Feed: | Standard food |
Animal Water: | Free access to food and water |
Species Group: | Mammal |
Select appropriate tab below to view additional metadata details:
Sample Preparation:
Sampleprep ID: | SP000947 |
Sampleprep Summary: | Briefly, for plasma samples, 95 µl of plasma was mixed with 5 µl of isotope standards mix and extracted with 400 µl of pre-cold extraction buffer containing methanol/acetonitrile (50/50, v/v). The mixture was incubated on ice for 30 min and centrifuged for 10 min at 16,000 g and 4 ºC. The supernatant containing both polar and nonpolar metabolites was transferred to new tubes and stored at -80 ºC for metabolomic analysis |
Extraction Method: | methanol/acetonitrile (50/50, v/v) precipitation |
Extract Storage: | -80 ºC |
Sample Derivatization: | No |
Sample Spiking: | Isotope standards |