Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA053487

Local Sample ID:	170608aOEsa19_1
Subject ID:	SU000948
Subject Type:	Human
Subject Species:	Homo sapiens
Taxonomy ID:	9606

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Subject
Factors
Collection
Treatment
Sample Preparation
Chromatography
Analysis
MS

Sample Preparation:

Sampleprep ID:	SP000955
Sampleprep Summary:	1) Aliquot 20 uL into 2 sample sets one for GC and one for LC analysis 2) Add 1 mL of 3:3:2 extraction solvent to ach tube 3) Vortex 10 sec 4) Shake 5 min 5) Centrifuge at 14000 RCF for 2 min 6) Aliquot 450 uL of supernatant into two sets of tubes saving one set for backup 7) Pipet the rest of sample into falcon tube 8) Dry down samples saving 1 set of backups 9) Aliquot 2 mL of the pooled samples from the falcon tube into 2 mL tubes then centrifuge again 10) Aliquot 450 uL of the pooled samples from the falcon tube into 1.5 mL tubes to dry down 11) Place pooled samples in centrivap to dry

Sampleprep ID:

SP000955

Sampleprep Summary:

1) Aliquot 20 uL into 2 sample sets one for GC and one for LC analysis 2) Add 1 mL of 3:3:2 extraction solvent to ach tube 3) Vortex 10 sec 4) Shake 5 min 5) Centrifuge at 14000 RCF for 2 min 6) Aliquot 450 uL of supernatant into two sets of tubes saving one set for backup 7) Pipet the rest of sample into falcon tube 8) Dry down samples saving 1 set of backups 9) Aliquot 2 mL of the pooled samples from the falcon tube into 2 mL tubes then centrifuge again 10) Aliquot 450 uL of the pooled samples from the falcon tube into 1.5 mL tubes to dry down 11) Place pooled samples in centrivap to dry