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MB Sample ID: SA055842

Local Sample ID:S_40_B
Subject ID:SU000978
Subject Type:Human
Subject Species:Homo sapiens
Taxonomy ID:9606

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Sample Preparation:

Sampleprep ID:SP000985
Sampleprep Summary:Study sample aliquots of 180 µL were mixed with 60 µL 0.9% saline solution containing 0.2% NaN3 and 4 mM formate in D2O. A total pool was also created by combining 11 µL aliquots from each study sample, mixed thoroughly, divided into four 180 µL aliquots, and processed identically to study samples. All of the samples were thoroughly mixed on multiple tube vortex mixer for 30 s at 5000 rpm, centrifuged at room temperature and 12,000 rcf for 5 min. The supernatants (200 µL) were stored in a fresh 1.5 mL centrifuge tubes overnight at 4 C, before transfer into pre-labeled 3mm NMR tubes (Bruker-Biospin, Switzerland) on the day of analysis.
Extract Storage:4℃
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