Return to study ST000972 main page
MB Sample ID: SA058598
| Local Sample ID: | S2_1 |
| Subject ID: | SU001011 |
| Subject Type: | Other |
| Subject Species: | Papio hamadryas |
| Taxonomy ID: | 9557 |
| Age Or Age Range: | 18 |
| Gender: | Male |
Select appropriate tab below to view additional metadata details:
Sample Preparation:
| Sampleprep ID: | SP001018 |
| Sampleprep Summary: | Aliquots of serum (30 µL) samples were subjected to sequential solvent extraction once each with 1 mL of acetonitrile: isopropanol: water (3:3:2) and 500 µL of acetonitrile: water (1:1) mixtures at 4 C.22 Adonitol and d4-succinic acid (both 5 µL from 10 mg/ml stock) were added to each aliquots as two internal standards prior to the extraction. The pooled extracts (~ 1500 µL) from the two steps were dried under vacuum at 4 C prior to chemical derivatization. Dummy extractions performed on blank tubes served as extraction blanks to account for background (extraction) noise and other sources of contamination. Six (S1, S2, S3, S6, S7, S8) samples were then sequentially derivatized with methoxyamine hydrochloride (MeOX) and 1% TMCS in N-methyl-N-trimethylsilyl-trifluoroacetamide (MSTFA) as described elsewhere.23,24 Steps involved addition of 10 μL of MeOX (20 mg mL-1) in pyridine incubated under shaking at 55 °C for 60 min followed by trimethylsilylation at 60 °C for 60 min after adding 90 μL MSTFA. |
| Processing Method: | Fiehn et al., 2008 |
| Processing Storage Conditions: | On ice |
| Extraction Method: | Fiehn et al., 2008 |
| Extract Enrichment: | None |
| Extract Cleanup: | None |
| Extract Storage: | -80℃ |
| Sample Derivatization: | Methoxyamination + silylation (MSTFA) |
| Sample Spiking: | Adonitol, d4-succinic acid |
