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MB Sample ID: SA060877
| Local Sample ID: | GLA_QEx_Lipids_SO069_SA068_1003eC3 |
| Subject ID: | SU001027 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
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Sample Preparation:
| Sampleprep ID: | SP001034 |
| Sampleprep Summary: | Extraction of plasma lipids was carried out using a biphasic solvent system of cold methanol, methyl tertbutyl ether (MTBE), and water with some modifications. In more detail, 300 μL of cold methanol containing a mixture of odd chain and deuterated lipid internal standards [LPE(17:1), LPC(17:0), PC(12:0/13:0), PE(17:0/17:0), PG(17:0/17:0), d7-cholesterol, SM(d18:1/17:0), Cer(d18:1/17:0), sphingosine (d17:1), DG(12:0/12:0/0:0), DG(18:1/2:0/0:0), and d5-TG(17:0/17:1/17:0)] was added to a 40 μL blood plasma aliquot in a 2 mL Eppendorf tube and then vortexed (10 s). Then, 1000 μL of cold MTBE containing CE 22:1 (internal standard) was added, followed by vortexing (10 s) and shaking (6 min) at 4 °C. Phase separation was induced by adding 250 μL of LC−MS grade water followed by centrifugation at 14000 rpm for 2 min. |
| Sampleprep Protocol Filename: | acs_analchem_7b03404.pdf |
