Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA071752

Local Sample ID:	1237A_RV
Subject ID:	SU001102
Subject Type:	Mammal
Subject Species:	Ovis aries
Taxonomy ID:	9940
Age Or Age Range:	142 days gestation (fetal); Within 24 hours of birth (newborn)

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Sample Preparation
Analysis
NMR

Sample Preparation:

Sampleprep ID:	SP001109
Sampleprep Summary:	Heart tissue (20-50 mg) was cut and weighed on dry ice before being placed on a sterile agar plate. Thirty μL of deuterium oxide (D2O) was added to each tissue sample before being placed in a 4 mm HR-MAS rotor (Bruker Biospin, Billerica, MA, USA) and leftover D2O (from original 30 μL) was added to the rotor with a pipette after placement of tissue. The samples were kept on dry ice until data acquisition.

Sampleprep ID:

SP001109

Sampleprep Summary:

Heart tissue (20-50 mg) was cut and weighed on dry ice before being placed on a sterile agar plate. Thirty μL of deuterium oxide (D2O) was added to each tissue sample before being placed in a 4 mm HR-MAS rotor (Bruker Biospin, Billerica, MA, USA) and leftover D2O (from original 30 μL) was added to the rotor with a pipette after placement of tissue. The samples were kept on dry ice until data acquisition.