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MB Sample ID: SA072355
| Local Sample ID: | Amb_R4_T2 |
| Subject ID: | SU001115 |
| Subject Type: | Plant |
| Subject Species: | Panicum maximum(Megathyrsis maximus (Jacq.)) |
| Taxonomy ID: | 59788 |
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Sample Preparation:
| Sampleprep ID: | SP001122 |
| Sampleprep Summary: | Room temp Methanol extration: 10-20 mg leaf material, extracted with 1.5 mL MeOH for 15 min at RT followed by another extraction with 1.4 mL H2O at RT. 1 mL of extraction dried under vacuum. 5 uL internal standard added to each sample. 1uL aliquot per sample |
| Sampleprep Protocol Comments: | As described in Ulanov, A. & J. M. Widholm (2010) Metabolic profiling to determine the cause of the increased triphenyltetrazolium chloride reduction in mannitol-treated maize callus. Journal of Plant Physiology, 167, 1423-1431. |
| Processing Method: | Homogenization and Solvent removal with speed vac |
| Processing Storage Conditions: | Room temperature |
| Extraction Method: | 1:1 MeOH/H20 |
| Extract Storage: | -80℃ |
| Sample Spiking: | Internal Standard (hentriacontanoic acid 5 uL) |
