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MB Sample ID: SA072432
| Local Sample ID: | HA_8 |
| Subject ID: | SU001117 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Genotype Strain: | Tcf/LacZ mice |
| Age Or Age Range: | 6-8 weeks |
| Gender: | Male and female |
| Animal Inclusion Criteria: | The Tcf/LacZ strain was previously backcrossed onto the C3H background C3H background, and confirmed by PCR to lack the rd1 mutation |
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Sample Preparation:
| Sampleprep ID: | SP001124 |
| Sampleprep Summary: | 4 mL of methanol (LC-MS grade) and 2 mL of chloroform (LC-MS grade) were added to each sample. After 2 min vigorous vortexing and 2 min sonication in ultrasonic bath, the samples were incubated at 48˚C overnight (in borosilicate glass vials, PTFE-lined caps). The following day, 2 mL of water (LC-MS grade) and 1 mL of chloroform were added, samples vigorously vortexed for 2 min and centrifuged at 3000 RCF, 4˚C for 15 min to obtain phase separation. Lower phases were collected and dried in centrifugal vacuum concentrator. Samples were stored at -20˚C until reconstituted in 150 µL of chloroform:methanol (1:1) prior to mass spectrometric analysis. Lipid profiling was performed in 2 batches: 7 day and 15 day period. For the 15 day period, quality control (QC) pooled sample was prepared and run 6 times throughout the batch. |
| Processing Method: | lipid extraction |
| Processing Storage Conditions: | Described in summary |
| Extraction Method: | chloroform, methanol, water phase separation |
| Extract Storage: | -80℃ |
| Sample Resuspension: | 150 µL of chloroform:methanol (1:1) |
| Sample Spiking: | no internal standards added |
