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MB Sample ID: SA075712
| Local Sample ID: | Heads Zt10 - Rep5_NFD |
| Subject ID: | SU001155 |
| Subject Type: | Insect |
| Subject Species: | Drosophila melanogaster |
| Taxonomy ID: | 7227 |
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Sample Preparation:
| Sampleprep ID: | SP001162 |
| Sampleprep Summary: | Add 1 mL of 3:3:2 Acetonitrile:Isopropyl Alcohol:LC-MS Grade Water to each preweighed sample. Vortex each sample for 5 seconds. Shake at 4°C for 5 minutes using the Orbital Mixing Chilling/Heating Plate. Keep remaining samples on ice before shaking. Centrifuge samples for 2 minutes at 14000 rcf. Aliquot two 450 µL portions of the supernatant. Submit one to analysis and save one as backup at -20°C. To the submitted sample, dry in Labconco Centrivap. Submit to derivatization. |
| Sampleprep Protocol Filename: | SOP_blood-GCTOF-11082012_332.pdf |
| Sampleprep Protocol Comments: | Extraction solvent is prepared previously. Begin procedure at Sample Preparation under step 3. |
| Sample Derivatization: | Add 10 µL of prepared MeOX solution to each sample. Shake for 1.5 hours at 30°C. Add 91 µL of prepared MSTFA + FAME solution to each sample. Shake for 30 minutes at 37°C. |
