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MB Sample ID: SA078040

Local Sample ID:BOSPT_1
Subject ID:SU001186
Subject Type:Bacteria
Subject Species:Bacteroides thetaiotaomicron VPI-5482;Bacteroides ovatus ATCC 8483
Taxonomy ID:226186;411476
Genotype Strain:WT (VPI-5482), SPT K.O. (BT_0870), (ATCC_8483), SPT K.O. (BACOVA_02588)
Age Or Age Range:411476
Cell Biosource Or Supplier:ATCC
Cell Strain Details:Wild type Bacteroides strains and serine palmitoyltransferase (SPT) knockouts
Subject Comments:Grown in BHI Media (Bacteroides tethaiotamicron, Bateroides ovatus : VPI-5482/ATCC_8483)

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Sample Preparation:

Sampleprep ID:SP001194
Sampleprep Summary:Bacterial cell pellets from 5ml overnight BHI cultures were resuspended in 5 mL isopropanol + 0.1 ng/μL C24:0 PC, incubated at RT for 1 hr, and centrifuged for 10 min at 10,000g.
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