Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA078346

Local Sample ID:	SM4-2
Subject ID:	SU001205
Subject Type:	Fungi
Subject Species:	Mortierella alpina
Taxonomy ID:	64518

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MS

Sample Preparation:

Sampleprep ID:	SP001213
Sampleprep Summary:	The obtained supernatants were vacuum dried at 30°C prior to derivatisation. The extraction solution served as a negative control to subtract from the background spectrum. The dried extract was derivatised. Briefly, the vacuum dried samples were resuspended in 100 μL of MeOX (10 mg/mL) in pyridine and incubated at 37°C for 90 min in a block heater. Then, 40 μL of MSTFA+1%TMCS was pipetted into each sample and incubated at 37°C for another 30 min. The resulting solution from each sample was collected and transferred into gas chromatography (GC) vials for analysis.

Sampleprep ID:

SP001213

Sampleprep Summary:

The obtained supernatants were vacuum dried at 30°C prior to derivatisation. The extraction solution served as a negative control to subtract from the background spectrum. The dried extract was derivatised. Briefly, the vacuum dried samples were resuspended in 100 μL of MeOX (10 mg/mL) in pyridine and incubated at 37°C for 90 min in a block heater. Then, 40 μL of MSTFA+1%TMCS was pipetted into each sample and incubated at 37°C for another 30 min. The resulting solution from each sample was collected and transferred into gas chromatography (GC) vials for analysis.