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MB Sample ID: SA080495
| Local Sample ID: | U41_A_4b |
| Subject ID: | SU001228 |
| Subject Type: | Other |
| Subject Species: | Acropora cervicornis |
| Taxonomy ID: | 6130 |
| Genotype Strain: | U25, U41, U44 |
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Sample Preparation:
| Sampleprep ID: | SP001236 |
| Sampleprep Summary: | Corol holobiont extract (in methonol) was added to double distilled water (1:2 v/v of sample to water), then flash freeze lyophilized (Labconco) until dry. Lyophilized dry powder was re-suspended in phosphate buffer in deuterium oxide at pH 7. The final volume for the 1H-NMR samples was 60 μL (in a 1.5 mm O.D. tube) with 90 % (v/v) of deuterated 50 mM sodium phosphate buffer (pH 7) with 2 mM of ethylene diamine tetra-acetic acid (EDTA). The remaining 10 % (v/v) was occupied by an internal standard [5 mM D6-4,4-dimethyl-4-silapentane-1-sulfonic acid (DSS-D6) and 0.2% sodium azide in deuterated environment; Chenomx, Inc.]. |
| Processing Method: | Lyophilization |
| Processing Storage Conditions: | -80℃ |
| Extract Storage: | -80℃ |
