Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA081611

Local Sample ID:	Young-2
Subject ID:	SU001245
Subject Type:	Mammal
Subject Species:	Mus musculus
Taxonomy ID:	10090
Genotype Strain:	wild type
Age Or Age Range:	8-week-old and 28-month-old
Weight Or Weight Range:	25.4 ± 0.2 g for young and 32.0 ± 0.2 g for aged
Gender:	Male
Animal Animal Supplier:	Shimizu Laboratory Supplies (Kyoto, Japan)
Animal Light Cycle:	12-h light-dark cycle
Animal Feed:	CE2
Species Group:	C57BL/6J

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Sample Preparation:

Sampleprep ID:	SP001253
Sampleprep Summary:	Gastrocnemius muscles of 8-week-old male mice (N=5, young group) and 28-month-old male mice (N=5, aged group) were used for the metabolomic analysis (Human Metabolome Technologies Inc., Tsuruoka, Japan). Frozen muscle specimens were transferred into 500 μL of methanol containing 50 mM external standard. After homogenization by BMSM10N21 (BMS, Tokyo) at 1,500 rpm for 120 s performed 5 times, 500 μL of chloroform and 200 μL of ultrapure water were added to the homogenate. The solution was mixed well and centrifuged at 2,300 × g for 5 min at 4 °C. The resultant water phase was ultrafiltered using a Millipore Ultrafree-MC PLHCC HMT Centrifugal Filter Device, 5 kDa (Millipore, Billerica, MA). The filtrate was dried and then dissolved in 50 μL of ultrapure water. The samples obtained were then subjected to CE-TOFMS analysis using the Agilent CE-TOFMS system (Agilent Technologies, Santa Clara, CA) at 4 °C. The detected peaks were aligned according to their m/z values and normalized migration times. The peaks were mean-centered and scaled using their standard deviations on a per peak basis as a pretreatment.

Sampleprep ID:

SP001253

Sampleprep Summary:

Gastrocnemius muscles of 8-week-old male mice (N=5, young group) and 28-month-old male mice (N=5, aged group) were used for the metabolomic analysis (Human Metabolome Technologies Inc., Tsuruoka, Japan). Frozen muscle specimens were transferred into 500 μL of methanol containing 50 mM external standard. After homogenization by BMSM10N21 (BMS, Tokyo) at 1,500 rpm for 120 s performed 5 times, 500 μL of chloroform and 200 μL of ultrapure water were added to the homogenate. The solution was mixed well and centrifuged at 2,300 × g for 5 min at 4 °C. The resultant water phase was ultrafiltered using a Millipore Ultrafree-MC PLHCC HMT Centrifugal Filter Device, 5 kDa (Millipore, Billerica, MA). The filtrate was dried and then dissolved in 50 μL of ultrapure water. The samples obtained were then subjected to CE-TOFMS analysis using the Agilent CE-TOFMS system (Agilent Technologies, Santa Clara, CA) at 4 °C. The detected peaks were aligned according to their m/z values and normalized migration times. The peaks were mean-centered and scaled using their standard deviations on a per peak basis as a pretreatment.