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MB Sample ID: SA082592
Local Sample ID: | B_cc_3 |
Subject ID: | SU001256 |
Subject Type: | Fungi |
Subject Species: | Ophiocordyceps sinensis;Cordyceps militaris |
Taxonomy ID: | 72228;73501 |
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Sample Preparation:
Sampleprep ID: | SP001264 |
Sampleprep Summary: | The extracts were centrifuged (15 min, 13,000 rpm) at 4oC and 3 mL of supernatant was collected. The supernatant was frozen at -80oC and then dried using a vacuum centrifugal evaporator (CHRiST, Alpha 2-4/LSC, Germany). The dried residues were separately dissolved in 3 mL of distilled water. A 600 µL extract was filtered (Millipore Amicon® ULTRA 3 kDa) and filtrates were collected. 3-(Trimethylsilyl) propanesulfonic acid (50 µL) pre-dissolved in D2O (an internal standard) was subsequently added to 450 µL of the filtrates. Finally, aliquots (480 µL) of each extract were transferred to a 5 mm NMR tube (Norell, Morganton, NC, USA) for NMR analysis. |