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MB Sample ID: SA082638
Local Sample ID: | J-83 |
Subject ID: | SU001257 |
Subject Type: | Animal |
Subject Species: | Sus scrofa |
Taxonomy ID: | 9823 |
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Sample Preparation:
Sampleprep ID: | SP001265 |
Sampleprep Summary: | The samples were crash deprotonized by methanol precipitation and spiked with D27-deuterated myristic acid (D27-C14:0) as an internal standard for retention-time locking and dried. The trimethylsilyl-D27-C14:0 standard retention time (RT) was set at 16.727 min. Reactive carbonyls were stabilized at 50C with methoxyamine hydrochloride in dry pyridine. Metabolites were made volatile with TMS groups using N-methyl-N-(trimethylsilyl) trifluoroacetamide or MSTFA with catalytic trimethylchlorosilane at 50C. |