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MB Sample ID: SA084748
| Local Sample ID: | 5h_DMSO_R1_troph_b |
| Subject ID: | SU001272 |
| Subject Type: | Cultured cells |
| Subject Species: | Plasmodium falciparum;Homo sapiens |
| Taxonomy ID: | 5833;9606 |
| Genotype Strain: | Cam3.IIR539T and Cam3.IIrev |
| Age Or Age Range: | 22-26 h post invasion |
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Sample Preparation:
| Sampleprep ID: | SP001280 |
| Sampleprep Summary: | RBCs infected with Cam3.IIR539T (resistant) or Cam3.IIrev (sensitive) P. falciparum parasites (22-26 h post invasion) were adjusted to 4% parasitaemia and 2% haematocrit and the culture medium refreshed prior to drug addition. Following the drug incubation period, 1E8 cells were pelleted by centrifugation at 1,000 x g for 3 min and the culture medium was removed. Parasite metabolism was quenched by the addition of ice-cold PBS, pelleted again and the supernatant discarded prior to metabolite extraction. Metabolites were extracted from the cell pellet using 150 µL of cold methanol. The extraction solvent containing the internal standard compounds CHAPS (3-[(3-cholamidopropyl)-dimethylammonio]-1-propanesulfonate), CAPS (3-(cyclohexylamino)-1-propanesulfonic acid), PIPES (1,4-piperazinediethanesulfonic acid) and TRIS (2-amino-2-(hydroxymethyl)-1,3-propanediol) was directly added to the cell pellet, mixed by pipetting and subjected to automatic vortex mixing for 1 h at 4°C. Following the 1 h incubation, samples were pelleted by centrifugation at 21,100 x g for 10 min, 110 µL of particle free supernatant was transferred to glass LC-MS vials and stored at -80°C until analysis. A 15 µL aliquot of each sample was combined to generate a pooled biological quality control (PBQC) sample. |
| Processing Storage Conditions: | Described in summary |
| Extract Storage: | Described in summary |
