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MB Sample ID: SA091029
| Local Sample ID: | 17_02 _t_minus_50 |
| Subject ID: | SU001319 |
| Subject Type: | Human |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
| Age Or Age Range: | 19-27 |
| Weight Or Weight Range: | 58-92 |
| Height Or Height Range: | 172-190 |
| Gender: | Male |
| Human Race: | Caucasian |
| Human Trial Type: | Prospective randomized open label controlled intervention study |
| Human Medications: | None |
| Human Prescription Otc: | None |
| Human Smoking Status: | Non smoker |
| Human Alcohol Drug Use: | Not in |
| Human Nutrition: | Fasted for 12 hours before the first sample was obtained. |
| Human Inclusion Criteria: | Healthy (normal physical examination, electrocardiography, and routine laboratory values). |
| Human Exclusion Criteria: | Febrile illness during the 2 weeks before the endotoxemia experiment, taking any prescription medication, history of spontaneous vagal collapse, practicing or experience with any kind of meditation, or participation in a previous trial where LPS was administered. |
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Sample Preparation:
| Sampleprep ID: | SP001327 |
| Sampleprep Summary: | Sample extraction was performed as described previously (https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0154597), with some modifications. Briefly, 50 µL of plasma was mixed with 450 µL of 90% (v/v) methanol containing internal standards and incubated for 15 min at 37°C with 1150 rpm. Precipitated proteins were separated from the extract by centrifugation for 12 min at 15000 rpm, after which supernatants were stored at -80 °C until further analysis. |
| Processing Storage Conditions: | -80℃ |
| Extraction Method: | Ethanol precipitation |
