Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA091503

Local Sample ID:	Fn-Sg_3
Subject ID:	SU001329
Subject Type:	Bacteria
Subject Species:	Fusobacterium nucleatum
Taxonomy ID:	190304
Genotype Strain:	Fusobacterium nucleatum subsp. nucleatum ATCC 25586
Cell Biosource Or Supplier:	ATCC 25586
Subject Comments:	Culture supernatants of Fusobacterium nucleatum subs. nucleatum ATCC 25586

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Sample Preparation:

Sampleprep ID:	SP001337
Sampleprep Summary:	To remove protein, 2 ml of chloroform and 0.8 ml of ultrapure water were added to the samples, which were thoroughly mixed and centrifuged at 2300 × g for 5 minutes at 4˚C. The upper aqueous layer was then transferred to ultrafilter tips (Amicon ultrafilter system™) and centrifuged at 9100 × g for 120 minutes at 4˚C. Filtered material was dried under reduced pressure, followed by suspension in 50 µl of ultrapure water.

Sampleprep ID:

SP001337

Sampleprep Summary:

To remove protein, 2 ml of chloroform and 0.8 ml of ultrapure water were added to the samples, which were thoroughly mixed and centrifuged at 2300 × g for 5 minutes at 4˚C. The upper aqueous layer was then transferred to ultrafilter tips (Amicon ultrafilter system™) and centrifuged at 9100 × g for 120 minutes at 4˚C. Filtered material was dried under reduced pressure, followed by suspension in 50 µl of ultrapure water.