Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA093379

Local Sample ID:	MTZC2350.1_t4
Subject ID:	SU001351
Subject Type:	Cultured cells
Subject Species:	Plasmodium falciparum
Taxonomy ID:	5833

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Sample Preparation:

Sampleprep ID:	SP001359
Sampleprep Summary:	The K13 mutant and WT ring-stage parasites were run in parallel for each metabolomic experiment to allow direct comparisons of their metabolomic states and the effects of DHA. Metabolites from saponin-lysed parasites were extracted with cold methanol along with spike-in control [13C4, 15N1]-Aspartate (Cambridge Isotope) as an internal LC/MS standard to correct for technical variation arising from sample processing in the data analysis phase, as described previously (Allman et al., 2016).Samples were injected on a Thermo Exactive Plus Orbitrap in negative ion mode (Lu, W. et al., Anal. Chem. 2010.).

Sampleprep ID:

SP001359

Sampleprep Summary:

The K13 mutant and WT ring-stage parasites were run in parallel for each metabolomic experiment to allow direct comparisons of their metabolomic states and the effects of DHA. Metabolites from saponin-lysed parasites were extracted with cold methanol along with spike-in control [13C4, 15N1]-Aspartate (Cambridge Isotope) as an internal LC/MS standard to correct for technical variation arising from sample processing in the data analysis phase, as described previously (Allman et al., 2016).Samples were injected on a Thermo Exactive Plus Orbitrap in negative ion mode (Lu, W. et al., Anal. Chem. 2010.).