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MB Sample ID: SA094356
Local Sample ID: | DDACS_ACLko_2_shield |
Subject ID: | SU001378 |
Subject Type: | Cultured cells |
Subject Species: | Toxoplasma gondii |
Taxonomy ID: | 5811 |
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Sample Preparation:
Sampleprep ID: | SP001386 |
Sampleprep Summary: | Pellets were washed with ice-cold PBS (3x) and metabolites were extracted in 250 μl chloroform:methanol:water (1:3:1). Samples were vortexed vigorously for 30 min at 4 °C and the insoluble material was pelleted through centrifugation (21,000g, 6 min, 4 °C). Samples were transferred to glass high-performance liquid chromatography (HPLC) vials and stored at −80 °C until analysis. |