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MB Sample ID: SA094698
| Local Sample ID: | CI_37 |
| Subject ID: | SU001388 |
| Subject Type: | Cultured cells |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
| Gender: | Male |
| Cell Primary Immortalized: | Islets isolated from pancreata of adult C57BL/J6 male mice |
| Cell Counts: | 100 islets |
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Sample Preparation:
| Sampleprep ID: | SP001396 |
| Sampleprep Summary: | With a randomized order, islet samples were put on dry ice before sample preparation. A volume of 400 uL ice cold methanol-water (80:20) was added, and then vortex for 5 min at 5000 rpm. All contents in the tube were transferred into pre-labeled MagNaLyzer tubes (with 10-15 beads inside). The tubes were put on bead homogenizer using quick run setting for bacterial cells (2 ml) with speed at 6.30 m/s for 45 sec in 1 cycle. All samples were centrifuged at 16,000 rcf for 20 min at 4°C. A volume of 300 µl supernatant were transferred into pre-labeled 1.5 ml Low-bind Eppendorf tube. For quality control purpose, 32 µl of the supernatant from individual sample was transferred and mixed in new 2-ml tube to make a sample pool (SP). A volume of 300 µl SP was transferred into a pre-labeled 2.0 ml Low-bind Eppendorf tubes. All samples, including study samples and pools, were dried under speed-vac. For immediate analysis, 100 µL of Water-Methanol (95:5) containing 500 ng/mL L-tryphtophan-d5 was added to the residue, and then vortexed for 10 mins at 5000 rpm. After centrifuge at 4°C, 16000 rcf for 4 min, the supernatant from individual sample was transferred to pre-labeled auto-sampler vial for LC-MS analysis. |
| Processing Storage Conditions: | -80℃ |
| Extract Storage: | -80℃ |
