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MB Sample ID: SA096545
| Local Sample ID: | Nip-24h-5 |
| Subject ID: | SU001405 |
| Subject Type: | Plant |
| Subject Species: | Oryza sativa Japonica Group |
| Taxonomy ID: | 39947 |
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Sample Preparation:
| Sampleprep ID: | SP001413 |
| Sampleprep Summary: | The rice panicle pre-cooled in liquid nitrogen were ground using a Mixer/mill (MM400; Retsch) with steel ball for 30 seconds at 30 HZ. Fifty milligram of rice panicle powder of each sample was extracted with a fixed volume (1 ml) of pre-cooled (−20 °C) extraction solvent (methanol: chloroform: water = 5: 2: 2) was added to homogenized tissues. After adding the extraction solvent, the vials/tubes were thoroughly vortexed for 1 min and then incubated on an orbital shaker (200 rpm) for 10 min at 4 °C followed by a 15 min sonication step. For phase separation, a volume of 500 µl of solvent (methanol: water = 1: 3), was added to each vial/tube and the samples were again thoroughly vortexed for 1 min. After that, the samples are centrifuged at a speed of 14000rpm for 10 min at 4 °C. one fixed volume 500 μL of the unpolar phase (the upper phase) was transferred into pre-labeled 1.5 ml microcentrifuge tube. Then the samples were dried in a SpeedVac concentrator without heating. The dried 500 μL aliquot from the upper phase for lipids profiling was re-suspended in 200 μL UPLC-grade ACN: 2-propanol: dichloromethane (1:1:1, v/v/v) and analyzed using LC-MS for lipidomics study. |
| Processing Storage Conditions: | -80℃ |
| Extract Storage: | -80℃ |
