Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA097765

Local Sample ID:	NT_WT_3
Subject ID:	SU001418
Subject Type:	Cultured cells
Subject Species:	Mus musculus
Taxonomy ID:	10090
Genotype Strain:	C57BL6/J
Age Or Age Range:	8-12
Gender:	Male

Select appropriate tab below to view additional metadata details:

All
Subject
Factors
Collection
Treatment
Sample Preparation
Chromatography
Analysis
MS

Sample Preparation:

Sampleprep ID:	SP001426
Sampleprep Summary:	A modified Bligh and Dyer extraction (Bligh and Dyer, 1959) was carried out on samples. Prior to biphasic extraction, a 13-lipid class Lipidyzer Internal Standard Mix is added to each sample (AB Sciex, 5040156). Following two successive extractions, pooled organic layers were dried down in a Genevac EZ-2 Elite. Lipid samples were resuspended in 1:1 methanol/dichloromethane with 10 mM Ammonium Acetate and transferred to robovials (Thermo 10800107) for analysis.

Sampleprep ID:

SP001426

Sampleprep Summary:

A modified Bligh and Dyer extraction (Bligh and Dyer, 1959) was carried out on samples. Prior to biphasic extraction, a 13-lipid class Lipidyzer Internal Standard Mix is added to each sample (AB Sciex, 5040156). Following two successive extractions, pooled organic layers were dried down in a Genevac EZ-2 Elite. Lipid samples were resuspended in 1:1 methanol/dichloromethane with 10 mM Ammonium Acetate and transferred to robovials (Thermo 10800107) for analysis.