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MB Sample ID: SA097991

Local Sample ID:Sample 33
Subject ID:SU001424
Subject Type:Yeast
Subject Species:Saccharomyces cerevisiae
Taxonomy ID:4932

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Sample Preparation:

Sampleprep ID:SP001432
Sampleprep Summary:Cell pellets were lyophilized to dryness overnight. The lyophilized pellet was homogenized in 50:50 acetonitrile/water with 0.3 % formic acid for OAs and AAs, 5% TCA for acetyl and malonyl CoA, 0.5M PCA for oxidized nucleotides and 50:50 methanol/NaOH for reduced nucleotides using the Precellys bead-based homogenizing system. Precellys tough micro-organism lysing kit (VK-05) was used to burst the yeast cells. An aliquot of homogenate for the required assay was aliquoted and immediately stored at -80oC.
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