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MB Sample ID: SA097991
Local Sample ID: | Sample 33 |
Subject ID: | SU001424 |
Subject Type: | Yeast |
Subject Species: | Saccharomyces cerevisiae |
Taxonomy ID: | 4932 |
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Sample Preparation:
Sampleprep ID: | SP001432 |
Sampleprep Summary: | Cell pellets were lyophilized to dryness overnight. The lyophilized pellet was homogenized in 50:50 acetonitrile/water with 0.3 % formic acid for OAs and AAs, 5% TCA for acetyl and malonyl CoA, 0.5M PCA for oxidized nucleotides and 50:50 methanol/NaOH for reduced nucleotides using the Precellys bead-based homogenizing system. Precellys tough micro-organism lysing kit (VK-05) was used to burst the yeast cells. An aliquot of homogenate for the required assay was aliquoted and immediately stored at -80oC. |