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MB Sample ID: SA098323
Local Sample ID: | 10040_T48 |
Subject ID: | SU001428 |
Subject Type: | Human |
Subject Species: | Homo sapiens |
Taxonomy ID: | 9606 |
Age Or Age Range: | 18-90+ |
Gender: | Male and female |
Human Race: | White, Black, Asian, Other |
Human Inclusion Criteria: | Criteria for inclusion were as follows: (1) patients 18 years or older with confirmed or presumed infection; (2) the presence of 2 or more systemic inflammatory response criteria; (3) enrollment within 24 hours of recognition of septic shock with initiation of a standardized sepsis treatment pathway; (4) the use of high-dose vasopressors (norepinephrine bitartrate >0.05 μg/kg/min, dopamine hydrochloride >10 μg/kg/min, phenylephrine hydrochloride >0.4 μg/kg/min, epinephrine >0.05 μg/kg/min, or any vasopressin dose) to treat shock for at least 4 hours at the time of enrollment; (5) cumulative SOFA score of at least 6; (6) and blood lactate level exceeding 18 mg/dL |
Human Exclusion Criteria: | . Patients were excluded if they were pregnant or breastfeeding or had any of the following characteristics: primary diagnosis other than sepsis, an established do-not-resuscitate status or advance directive restricting aggressive care, any history of seizures, known inborn error of metabolism, anticipated surgery that would interfere with a 12-hour infusion, active participation in another interventional trial, cardiopulmonary resuscitation before enrollment, known allergy to levocarnitine, active warfarin treatment, or severe immunocompromised state |
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Sample Preparation:
Sampleprep ID: | SP001436 |
Sampleprep Summary: | Methanol precipitation, ultrafiltration |
Sampleprep Protocol ID: | Methanol precipitation, Ultrafiltration |
Sampleprep Protocol Filename: | 235.01-Serum_MeOH_Precip_and_Resuspension.pdf Expt._235.1_Filtration_of_CaPS_RACE_Serums.pdf |
Processing Method: | Methanol precipitation, dried by lyophilization, ultrafiltered |
Processing Storage Conditions: | On ice |
Extraction Method: | Methanol precipitation |
Extract Enrichment: | Lyophilization |
Sample Resuspension: | 500 uL 50mM sodium phosphate buffer in Deuterium Oxide (D2O) |
Sample Spiking: | CaFormate internal standard |