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MB Sample ID: SA099879
| Local Sample ID: | WT5 |
| Subject ID: | SU001447 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
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Sample Preparation:
| Sampleprep ID: | SP001455 |
| Sampleprep Summary: | All processes are carried out on ice. An aliquot (1 mL) of 80% MeOH extraction solvent is added to the sample (with Internal Standards spiked in) for protein precipitation. The 80% MeOH extraction solvent is precooled to -80C for at least one hour. After addition of the extraction solvent, the samples are then placed in the -80 oC freezer for 30 minutes. After incubation, the samples are immediately centrifuged at 18,800 × g (Microfuge 22R, Beckman Coulter) at 4 oC for 10 minutes. After centrifugation, the supernatant is transferred to new a microcentrifuge tube (Eppendorf brand) for drying by vacuum concentration. The dried metabolites are re-dissolved in 80% MeOH. The precipitated protein pellet is resolubilized for Bradford assays to measure the protein concentration as a quality control for the sample loading. |
| Processing Storage Conditions: | On ice |
| Extraction Method: | Methanol |
| Extract Enrichment: | Vacuum centrifugation |
| Sample Resuspension: | 80% MeOH |
| Sample Derivatization: | None |
| Sample Spiking: | Internal Standards added |
| Subcellular Location: | N/A |
