Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA102791

Local Sample ID:	6246246
Subject ID:	SU001460
Subject Type:	Human
Subject Species:	Homo sapiens
Taxonomy ID:	9606

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Sample Preparation:

Sampleprep ID:	SP001468
Sampleprep Summary:	Blood plasma or serum is extracted following the protocols published e.g. in Fiehn et al. PLoS ONE (2010) e15234. 30 ?L aliquots are extracted by 1 mL of degassed acetonitrile : isopropanol : water (3:3:2,v/v/v ) at –20°C, centrifuged and decanted with subsequent evaporation of the solvent to complete dryness. A clean-up step with acetonitrile/water (1:1) removes membrane lipids and triglycerides. The cleaned extract is aliquoted into two equal portions and the supernatant is dried down again. Internal standards C08-C30 FAMEs are added and the sample is derivatized by methoxyamine hydrochloride in pyridine and subsequently by N-methyl-N-trimethylsilyltrifluoroacetamide for trimethylsilylation of acidic protons.

Sampleprep ID:

SP001468

Sampleprep Summary:

Blood plasma or serum is extracted following the protocols published e.g. in Fiehn et al. PLoS ONE (2010) e15234. 30 ?L aliquots are extracted by 1 mL of degassed acetonitrile : isopropanol : water (3:3:2,v/v/v ) at –20°C, centrifuged and decanted with subsequent evaporation of the solvent to complete dryness. A clean-up step with acetonitrile/water (1:1) removes membrane lipids and triglycerides. The cleaned extract is aliquoted into two equal portions and the supernatant is dried down again. Internal standards C08-C30 FAMEs are added and the sample is derivatized by methoxyamine hydrochloride in pyridine and subsequently by N-methyl-N-trimethylsilyltrifluoroacetamide for trimethylsilylation of acidic protons.