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MB Sample ID: SA113203

Local Sample ID:224_025
Subject ID:SU001464
Subject Type:Mammal
Subject Species:Sus scrofa
Taxonomy ID:9823

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Sample Preparation:

Sampleprep ID:SP001472
Sampleprep Summary:Whole blood samples were thawed on ice and subjected to a methanol-chloroform precipitation. Briefly, samples were thawed in an ice-water bath after which 500 μL of blood was transferred to a microcentrifuge tube and 1ml of a 1:1 methanol-chloroform solution was added. Samples were sonicated for 2 minutes at 4°C, then incubated at -20°C for 20 minutes, and then centrifuged (13,400g at 4°C for 30 minutes). The aqueous supernatant was transferred to a new microcentrifuge tube and dried by lyophilization. Samples were then resuspended in 600 μL of 50mM sodium phosphate buffer in D2O for NMR analysis.
Processing Storage Conditions:On ice