Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA113470

Local Sample ID:	Evocrp5_1
Subject ID:	SU001468
Subject Type:	Bacteria
Subject Species:	Escherichia coli
Taxonomy ID:	562
Genotype Strain:	K-12 MG1655

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Sample Preparation:

Sampleprep ID:	SP001476
Sampleprep Summary:	Samples were extracted from the Wildtype, crp mutant and the five evolved replicate population, grown in a bioreactor aerobically in glucose minimal media with 40 mM MOPS at 37 degrees Celsius and 700 rpm. The metabolites were extracted using the Methanol-Chloroform-Water method. The samples were spiked with an equal volume of 13C-labelled internal standard taken from the same batch at an earlier stage to prevent to account for losses due to metabolite degradation and to enable robust quantification circumventing the ion-suppression effects.

Sampleprep ID:

SP001476

Sampleprep Summary:

Samples were extracted from the Wildtype, crp mutant and the five evolved replicate population, grown in a bioreactor aerobically in glucose minimal media with 40 mM MOPS at 37 degrees Celsius and 700 rpm. The metabolites were extracted using the Methanol-Chloroform-Water method. The samples were spiked with an equal volume of 13C-labelled internal standard taken from the same batch at an earlier stage to prevent to account for losses due to metabolite degradation and to enable robust quantification circumventing the ion-suppression effects.