Metabolomics Workbench : NIH Data Repository

MB Sample ID: SA113835

Local Sample ID:	mct1KO at 3hr minR+Leu Sample6
Subject ID:	SU001475
Subject Type:	Yeast
Subject Species:	Saccharomyces cerevisiae
Taxonomy ID:	4932
Genotype Strain:	BY4743
Gender:	Not applicable

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Sample Preparation:

Sampleprep ID:	SP001483
Sampleprep Summary:	To each sample was added boiling 75% ethanol (EtOH) solution containing the internal standard d4-succinic acid (Sigma 293075). Boiling samples were vortexed and incubated at 90°C for 5 min. Samples were then incubated at -20 ˚C for 1 hr. After incubation the samples were centrifuged at 5,000 x g for 10 minutes at 4˚C. The supernatant was then transferred from each sample tube into a labeled, fresh 13x100mm glass culture tube. A second standard was then added (d27-myristic acid CDN Isotopes: D-1711). Pooled quality control samples were made by removing a fraction of collected supernatant from each sample and process blanks were made using only extraction solvent and no cell culture. The samples were then dried en vacuo. This process was completed in three separate batches.

Sampleprep ID:

SP001483

Sampleprep Summary:

To each sample was added boiling 75% ethanol (EtOH) solution containing the internal standard d4-succinic acid (Sigma 293075). Boiling samples were vortexed and incubated at 90°C for 5 min. Samples were then incubated at -20 ˚C for 1 hr. After incubation the samples were centrifuged at 5,000 x g for 10 minutes at 4˚C. The supernatant was then transferred from each sample tube into a labeled, fresh 13x100mm glass culture tube. A second standard was then added (d27-myristic acid CDN Isotopes: D-1711). Pooled quality control samples were made by removing a fraction of collected supernatant from each sample and process blanks were made using only extraction solvent and no cell culture. The samples were then dried en vacuo. This process was completed in three separate batches.