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MB Sample ID: SA114130
| Local Sample ID: | LIPNEG17MAR_09.38 |
| Subject ID: | SU001479 |
| Subject Type: | Cultured cells |
| Subject Species: | Homo sapiens |
| Taxonomy ID: | 9606 |
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Sample Preparation:
| Sampleprep ID: | SP001487 |
| Sampleprep Summary: | Cells were pelleted and processed using a chloroform:methanol homogenization followed by an isoproponal:acetonitrile extraction. Samples were separated by reverse phase HPLC using a Prominence 20 UFLCXR system (Shimadzu, Columbia MD) with a Waters (Milford, MA) CSH C18 column (100mm x 2.1mm 1.7 um particle size) maintained at 55C and a 20 minute aqueous/acetonitrile/isopropanol gradient, at a flow rate of 225 ul/min. For electrospray ionization positive mode, Solvent A was 40% water, 60% acetonitrile with 10mM ammonium formate and 0.1% formic acid, and Solvent B was 90% isopropanol, 10% acetonitrile with 10mM ammonium formate and 0.1% formic acid. For electrospray ionization negative mode, Solvent A was 40% water, 60% isopropanol with 10 mM ammonium acetate, and solvent B was 90% isopropanol, 10% acetonitrile with 10 mM ammonium acetate. The initial conditions were 60% A and 40% B, increasing to 43% B at 2 minutes, 50% B at 2.1 minutes., 54% B at 12 minutes, 70% B at 12.1 minutes and 99% B at 18 minutes, held at 99% B until 20.0 minutes before returning to the initial conditions. |
| Processing Storage Conditions: | -80℃ |
| Extract Storage: | -80℃ |
