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MB Sample ID: SA124517
| Local Sample ID: | 26_B1-1_Lung_syngenic_7days_170427_UKy_GCH_rep2-polar-ICMS_A |
| Subject ID: | SU001544 |
| Subject Type: | Mammal |
| Subject Species: | Mus musculus |
| Taxonomy ID: | 10090 |
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Sample Preparation:
| Sampleprep ID: | SP001552 |
| Sampleprep Summary: | Protein extraction and quantification. Before going into the IC-FTMS the frozen sample is reconstituted in water. Tissue is frozen in liquid nitrogen to stop metabolic processes. Polar extraction from homogenate, lypholized, and frozen. Frozen tissue is ground in a SPEX grinder under liquid nitrogen to homogenize the sample. |
| Sampleprep Protocol ID: | IC-FTMS_preparation; polar_extraction; tissue_quench; protein_extraction; frozen_tissue_grind |
| Sampleprep Protocol Filename: | 4B_Extract_Polar_Lipid_Prot_Fan_070417.pdf 4D_17Jun4_Fan_Prot_Quant.pdf |
