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MB Sample ID: SA126303
| Local Sample ID: | Spike_010_5 |
| Subject ID: | SU001572 |
| Subject Type: | Bacteria |
| Subject Species: | Fecal Bacteria |
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Sample Preparation:
| Sampleprep ID: | SP001580 |
| Sampleprep Summary: | Fecal supernatants were serially diluted using 50% acetonitrile and 0.1% formic acid in water to obtain final dilution of 1:10,000. 500 µl of each sample were placed in glass vials (Wicom, Heppenheim, Germany) and stored at - 80 °C until LC-MS/MS analysis.For the analysis of sulfonates by LC-MS/MS-MRM, aliquots of 250 µl for each sample were centrifuged (14 000 x g, 4 °C, 5 min) and 50 µl of the supernatant stored at – 20 °C until further processing. Samples were thawed, centrifuged at 18 000 x g at RT for 2 min and the supernatant was diluted 1:10 000 in 50% aqueous acetonitrile. Subsequently, 500 µl of each sample were placed in glass vials (Wicom, Heppenheim, Germany) and stored at - 80 °C until LC-MS/MS analysis. |
| Sampleprep Protocol Filename: | sven_haange_Sample_preparation-application_experiment.pdf |
